Gene Expression Changes After Ventral Root Replantation
-
Christopher
- Posts: 845
- Joined: Wed Jun 18, 2003 10:09 pm
- Injury Description, Date, extent, surgical intervention etc: Date of Injury: 12/15/02
Level of Injury:
-dominant side C5, C6, & C7 avulsed. C8 & T1 stretched & crushed
BPI Related Surgeries:
-2 Intercostal nerves grafted to Biceps muscle,
-Free-Gracilis muscle transfer to Biceps Region innervated with 2 Intercostal nerves grafts.
-2 Sural nerves harvested from both Calves for nerve grafting.
-Partial Ulnar nerve grafted to Long Triceps.
-Uninjured C7 Hemi-Contralateral cross-over to Deltoid muscle.
-Wrist flexor tendon transfer to middle, ring, & pinky finger extensors.
Surgical medical facility:
Brachial Plexus Clinic at The Mayo Clinic, Rochester MN
(all surgeries successful)
"Do what you can, with what you have, where you are."
~Theodore Roosevelt - Location: Los Angeles, California USA
Gene Expression Changes After Ventral Root Replantation
http://www.frontiersin.org/neurotrauma/ ... 9/abstract
Frontiers IN Neurotrauma
On Acute Gene Expression Changes After Ventral Root Replantation
Marten Risling1*, Thomas Ochsmann1, Thomas Carlstedt2, Hans Lindå1, Stefan Plantman1, Elham Rostami1, Maria Angeria1 and Mattias Sköld3
1 2 3
Neuroscience, Karolinska institutet, Sweden Handsurgery, Södersjukhuset, Sweden Section of Neurosurgery, Uppsala University Hospital, Sweden
Replantation of avulsed spinal ventral roots has been show to enable significant and useful regrowth of motor axons in both experimental animals and in human clinical cases, making up an interesting exception to the rule of unsuccessful neuronal regeneration in CNS. Compared to avulsion without repair, ventral root replantation seems to rescue lesioned motoneurons from death. In this study we have analyzed the acute response to ventral root avulsion and replantation in adult rats with gene arrays combined with cluster analysis of gene ontology search terms. The data show significant differences between rats subjected to ventral replantation compared to avulsion only. Even though number of genes related to cell death is similar in the two models after 24 hours, we observed a significantly larger number of genes related to neurite growth and development in the rats treated with ventral root replantation, possibly reflecting the neuroregenerative capacity in the replantation model. In addition, an acute inflammatory response was observed after avulsion, while effects on genes related to synaptic transmission were much more pronounced after replantation than after avulsion alone. These data indicate that the axonal regenerative response from replantation is initiated at an earlier stage than the possible differences in terms of neuron survival. We conclude that this type of analysis may facilitate the comparison of the acute response in two types of injury.
Keywords: Gene expression, Inflammation, Regeneration, Spinal cord, Ventral root Citation: Risling M, Ochsmann T, Carlstedt T, Lindå H, Plantman S, Rostami E, Angeria M and Sköld M (2010). On Acute Gene Expression Changes After Ventral
Root Replantation. Front. Neur. doi: 10.3389/fneur.2010.00159
Received: 08 Sep 2010; Accepted: 21 Dec 2010.
Edited by: Jia Lu, DSO National Laboratories, Singapore
Reviewed by: Eng-Ang Ling, National University of Singapore, Singapore Bei-Ping He, National University of Singapore, Singapore
Copyright: © 2010 Risling, Ochsmann, Carlstedt, Lindå, Plantman, Rostami, Angeria and Sköld. This is an open-access publication subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.
* Correspondence: Dr. Marten Risling, Karolinska institutet, Neuroscience, Retzius väg 8, Stockholm, S-17177, Sweden, Marten.Risling@ki.se
